ABSTRACT
Significant risks to human health are posed by the 2019 coronavirus illness (COVID-19). SARS coronavirus type 2 receptor, also known as the major enzyme in the renin-angiotensin system (RAS), angiotensin-converting enzyme 2 (ACE-2), connects COVID-19 and RAS. This study was conducted with the intention of determining whether or not RAS gene polymorphisms and ACE-2 (G8790A) play a part in the process of predicting susceptibility to infection with COVID-19. In this study 127 participants, 67 of whom were deemed by a physician to be in a severe state of illness, and 60 of whom were categorized as "healthy controls".The genetic study included an extraction of genomic DNA from blood samples of each covid 19 patients and healthy controls, then amplification the site of SNP (rs2285666) Within the ACE2 gene by using specific primers, sequencing PCR products, and genotyping to detect the role of the ACE-2 gene (rs2285666) in the incidence of COVID-19. ACE-2 (rs2285666) is statistically associated to COVID-19. The COVID-19 group had 65.67 %of individuals with the wild-type homozygous genotype (GG) and 20% in the control group, while the control group had 63.33% of individuals with the mutant genotype (AA). Consequently, the wild-type homozygous (GG) and allele (G) may be considered a risk factor (etiological fraction E. F) for COVID-19 in Iraqi patients, whereas the mutant homozygous (AA) and allele (A) may be considered a protective factor (preventive fraction). The findings of the present study reveal that carriers of the GG genotype of ACE2 (rs2285666) are substantially more susceptible to COVID-19.Copyright © 2023 Allami et al.
ABSTRACT
COVID-19 disease is a highly contagious respiratory inflammation occur by viral infection (SARS-CoV-2). Cytokines are proteins that mediators of dysregulated immunity, and stimulated of immune response such as interlukin-6 (IL-6). Our study included two hundred and twenty samples of COVID 19 patients of Iraqi population, their ages ranged from 21 to 79 year (mean of ages 37.8 ± 1.4) and one hundred and twenty of the controls (outwardly healthy), their ages ranged from 19 to 54 year (mean of ages 27.9 ± 1.9). We confined the frequency of IL-6 Gene polymorphism by using Tetra-amplification refractory mutation system-polymerase chain reaction technique (TARMS PCR). Also, we determined the association of IL-6 Gene polymorphism in position (-174 G/C) with COVID 19 for Iraqi community. Data showed significant difference in genotypesfrequency of IL-6 Gene polymorphism with COVID-19 patients and control. The genotype of GG (homozygotes) show high frequency in COVID 19 patients was ratio 63.64%, comparison with control was 47.50% and showed related with etiological fraction risk of SARS-CoV-2 ratio was 30.7%, and OR (CI 95%) was 1.63(1.23 to 3.03). The genotypes of GC (Heterozygotes) show high frequency in control ratio was 50%, while in COVID-19 patients show low frequency was 32.72%, also GC genotypes showed relationship with preventive fraction risk of SARS-CoV-2 ratio was 25.7% and OR (CI 95%) was 0.49 (0.31 to 0.77). The CC genotype show non-significant between COVID-19 patients and control and ratio was 3.64% and 2.50% respectively, and was OR (CI 95%) 1.47 (0.38 TO 5.63). The G allele show high in frequency of COVID-19 patients comparison with control ratio was 80% and 72.50% respectively, and present with etiological fraction risk of SARS-CoV-2, while C allele show low frequency in COVID-19 patients comparison with control ratio was 27.50% and 20% respectively, and present related with preventive fraction for SARS-CoV-2. Our findings demonstrate that the IL-6 gene in position -174 G/C may represent of SARS-CoV-2 risk development of COVID-19 patient’s in Iraqi population. © 2022, ResearchTrentz Academy Publishing Education Services. All rights reserved.
ABSTRACT
COVID-19 disease is a highly contagious respiratory inflammation occur by viral infection (SARS-CoV-2). Cytokines are proteins that mediators of dysregulated immunity, and stimulated of immune response such as interlukin-6 (IL-6). Our study included two hundred and twenty samples of COVID 19 patients of Iraqi population, their ages ranged from 21 to 79 year (mean of ages 37.8 + 1.4) and one hundred and twenty of the controls (outwardly healthy), their ages ranged from 19 to 54 year (mean of ages 27.9 ± 1.9). We confined the frequency of IL-6 Gene polymorphism by using Tetra-amplification refractory mutation system-polymerase chain reaction technique (TARMS PCR). Also, we determined the association of IL-6 Gene polymorphism in position (-174 G/C) with COVID 19 for Iraqi community. Data showed significant difference in genotypes frequency of IL-6 Gene polymorphism with COVID-19 patients and control.The genotype of GG (homozygotes) show high frequency in COVID 19 patients was ratio 63.64%, comparison with control was 47.50% and showed related with etiological fraction risk of SARS-CoV-2 ratio was 30.7%, and OR (Cl 95%) was 1.63(1.23 to 3.03). The genotypes of GC (Heterozygotes) show high frequency in control ratio was 50%, while in COVID-19 patients show low frequency was 32.72%, also GC genotypes showed relationship with preventive fraction risk of SARS-CoV-2 ratio was 25.7% and OR (Cl 95%) was 0.49 (0.31 to 0.77). The CC genotype show non-significant between COVID-19 patients and control and ratio was 3.64% and 2.50% respectively, and was OR (Cl 95%) 1.47 (0.38 TO 5.63). The G allele show high in frequency of COVID-19 patients comparison with control ratio was 80% and 72.50% respectively, and present with etiological fraction risk of SARS-CoV-2, while C allele show low frequency in COVID-19 patients comparison with control ratio was 27.50% and 20% respectively, and present related with preventive fraction for SARS-CoV-2. Our findings demonstrate that the IL-6 gene in position -174 G/C may represent of SARS-CoV-2 risk development of COVID-19 patient's in Iraqi population © 2022, ResearchTrentz Academy Publishing Education Services. All rights reserved.